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KMID : 0359320030430040641
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Korean Journal of Veterinary Research
2003 Volume.43 No. 4 p.641 ~ p.648
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Effects of HgCl₂ on plasma DNA content and blood biochemical values in rats
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조준형/Cho, Joon-Hyoung
정상희/강환구/윤효인/Jeong, Sang-Hee/Kang, Hwan-Goo/Yun, Hyo-In
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Abstract
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Changes of plasma DNA contents and serum biochemical values were measured in rats administered with HgCI_(2) to investigate the in vivo cytotoxic effects of mercury and examine the usefulness of these changes as indicators of mercury exposure and diagnosis of mercury poisoning. Rats were given once intraperitonealy HgCI_(2)(0.13, 0.32, 0.8 and 2 mg/kg b.w) and the changes of plasma DNA contents and serum biochemical values were measured at the time of 2, 4, 8, 24, 48 and 72 hours after the administration of HgCI(2-) Plasma DNA contents began to increase from 2 hours after the administration of HgCI_(2) in all the treatment groups significantly compared to control with dose-dependent pattern. The levels of plasma DNA reached to peak at 48 hours as 2.77, 7.60, 15.46 and 16.51 times higher than control in each treatment group of 0.13, 0.32, 0.8 and 2 mg.kgb.w, respectively and remained to be higher until 72 hours after the administration. The values of creatine kinase, aspartate aminotransferase, alanine aminotransferase, lactate dehydrogenase, blood urea nitrogen and glucose of serum were increased, however the values of alkaline phosphatase, total protein and triglyceride were decreased. These changes of increase and decrease showed dose-dependent pattern but the starting time, maintenance and magnitude of change were various and characteristic according to serum biochemical indices. Among the changes of serum biochemical values, those of aspartate aminotransferase, lactate dehydrogenase and blood urea nitrogen were apparently and significantly increased compared to control from 2 to 72 hours by the administration of 2 mg/kg HgCI_(2). This study demonstrates that plasma DNA and serum biochemical values such as aspartate aminotransferase, lactate dehydrogenase, blood urea nitrogen and etc. are valuable as biomarkers for mercury exposure assessment and diagnosis of mercury poisoning.
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KEYWORD
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